Journal: Medicines

Article Title: Analgesic Efficacy of a Combination of Fentanyl and a Japanese Herbal Medicine “ Yokukansan ” in Rats with Acute Inflammatory Pain

doi: 10.3390/medicines7120075

Figure Lengend Snippet: The component galenicals of Yokukansan (YKS).

Article Snippet: The three-dimensional high-performance liquid chromatography (3D-HPLC) profile chart of YKS was provided by Tsumura & Co. ( ).

Techniques:

Journal: Medicines

Article Title: Analgesic Efficacy of a Combination of Fentanyl and a Japanese Herbal Medicine “ Yokukansan ” in Rats with Acute Inflammatory Pain

doi: 10.3390/medicines7120075

Figure Lengend Snippet: Three-dimensional high-performance liquid chromatography (3D-HPLC) profile chart of the major chemical compounds in Yokukansan (YKS).

Article Snippet: The three-dimensional high-performance liquid chromatography (3D-HPLC) profile chart of YKS was provided by Tsumura & Co. ( ).

Techniques: High Performance Liquid Chromatography

Journal: Medicines

Article Title: Analgesic Efficacy of a Combination of Fentanyl and a Japanese Herbal Medicine “ Yokukansan ” in Rats with Acute Inflammatory Pain

doi: 10.3390/medicines7120075

Figure Lengend Snippet: Duration of pain-related behavior with the formalin test. The combination of Yokukansan (YKS) and fentanyl (FEN) significantly inhibited pain-related behavior during the early phase (0–10 min after formalin injection) and the intermediate phase (10–20 min). The duration in the YKS group was significantly shorter compared with the FEN group during the late phase. FOR, formalin-injected group; YKS, YKS-treated+FOR group; FEN, fentanyl-treated+FOR group; YKS+FEN, YKS+FEN+FOR group. Mean ± SD. ## p < 0.01 (vs. control), * p < 0.05, ** p < 0.01 (Tukey–Kramer test).

Article Snippet: The three-dimensional high-performance liquid chromatography (3D-HPLC) profile chart of YKS was provided by Tsumura & Co. ( ).

Techniques: Injection, Control

Journal: Medicines

Article Title: Analgesic Efficacy of a Combination of Fentanyl and a Japanese Herbal Medicine “ Yokukansan ” in Rats with Acute Inflammatory Pain

doi: 10.3390/medicines7120075

Figure Lengend Snippet: Pharmacological effects of formalin, fentanyl, and Yokukansan on TRPA1. Typical traces (left) and current–voltage relationships (right) of formalin-induced TRPA1 currents in HEK293T cells expressing human TRPA1. The concentrations of formalin (For), fentanyl (FEN), and Yokukansan (YKS) were 0.003%, 10 μM, and 1 mg/mL, respectively. YKS ( a ) or FEN ( b ), and YKS and FEN ( c ) were pretreated for 1 min before the concomitant administration of formalin. TRPA1 was not inhibited by both fentanyl and YKS. The holding potential was −60 mV, and ramp pulses (−100 to +100 mV, 300 ms) were applied every 5 s.

Article Snippet: The three-dimensional high-performance liquid chromatography (3D-HPLC) profile chart of YKS was provided by Tsumura & Co. ( ).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Saireito (TJ-114), a Japanese Traditional Herbal Medicine, Reduces 5-Fluorouracil-Induced Intestinal Mucositis in Mice by Inhibiting Cytokine-Mediated Apoptosis in Intestinal Crypt Cells

doi: 10.1371/journal.pone.0116213

Figure Lengend Snippet: 5-FU (50 mg/kg) was injected i.p. once daily while saireito (100–1000 mg/kg) and daikenchuto (2700 mg/kg) were administered p.o. twice daily for 6 days (days 0–5). Body weight is shown as a percentage of initial body weight (A), whereas the severity of diarrhea is scored using the four-grade scale (0 to 3) (B). Data are presented as the mean ± SEM of 6–8 mice. * P < 0.05 versus control (vehicle alone), # P < 0.05 versus normal (5-FU-untreated).

Article Snippet: Daikenchuto extract is prepared from a decoction of the following three medicinal herbs: Zanthoxylum Fruit , 2.0 g; Ginseng , 3.0 g; and processed Ginger , 5.0 g. The three-dimensional high-performance liquid chromatograph (3D-HPLC) profile of saireito provided by Tsumura & Co. is shown in .

Techniques: Injection

Journal: PLoS ONE

Article Title: Saireito (TJ-114), a Japanese Traditional Herbal Medicine, Reduces 5-Fluorouracil-Induced Intestinal Mucositis in Mice by Inhibiting Cytokine-Mediated Apoptosis in Intestinal Crypt Cells

doi: 10.1371/journal.pone.0116213

Figure Lengend Snippet: 5-FU (50 mg/kg) was injected i.p. once daily while saireito (100–1000 mg/kg) and daikenchuto (2700 mg/kg) were administered p.o. twice daily for 6 days (days 0–5). The jejunum was excised on day 6, sectioned, and stained with H&E. Histological observations for intestinal villi (A, 100×) and crypts (B, 400×) were performed. The height of villi (C), the number of surviving crypts per millimeter (D), and surviving cells per crypt (E) were measured. Data are presented as the mean ± SEM of 6–8 mice. * P < 0.05 , versus control (vehicle alone); # P < 0.05 , versus normal (5-FU-untreated).

Article Snippet: Daikenchuto extract is prepared from a decoction of the following three medicinal herbs: Zanthoxylum Fruit , 2.0 g; Ginseng , 3.0 g; and processed Ginger , 5.0 g. The three-dimensional high-performance liquid chromatograph (3D-HPLC) profile of saireito provided by Tsumura & Co. is shown in .

Techniques: Injection, Staining